A recently published article in the Journal of Chromatography B (L. De Colli, C. Elliott, J. Finnan, J. Grant, E.K. Arendt, S.P. McCormick, M. Danaher, Determination of 42 mycotoxins in oats using a mechanically assisted quechers sample preparation and UHPLCMS/MS detection, Journal of Chromatography B (2020), doi: https://doi.org/10.1016/j.jchromb.2020.122187) featured a developed and validated method for the simultaneous determination of 42 mycotoxins in oats. The assessed panel includes all the mycotoxins listed under Commission Regulation 1881/2006 and Commission Recommendation 165/2013, the emerging mycotoxins (beauvericin, alternariol, alternariol-methyl-ether and enniatins), and two masked metabolites, namely deoxynivalenol-3-glucoside and T-2-glucoside. The method also focuses on a wide range of analytes of toxicological interest.
The sample preparation involved extraction with an aqueous acetic acid solution and acetonitrile, followed by a QuEChERS technique (UCT Anhydrous Magnesium Sulphate (MgSO4) and Sodium Chloride (NaCl) blend) with mechanically assisted vibrational shaking.
Highlighting the high degree of accuracy and sample throughput provided, this approach can be applied to a large number of compounds currently not regulated, thus generating knowledge and for risk assessment purposes. In addition, the research highlights the need for multi-mycotoxin methods that include regulated, emerging and modified mycotoxins all in one test.