The kidneys are considered critical for the long-term control of blood pressure by regulation of body fluid and blood volume. In support of this concept, the kidneys control the systemic activity of the pro-hypertensive renin-angiotensin-aldosterone system by regulating the production and release of renin from juxtaglomerular cells.
The relationship between the endocannabinoid system in the renal medulla and the long-term regulation of blood pressure is not yet completely understood. In a recent paper authored by Zdravka Daneva et al., (Journal of Pharmacology and Experimental Therapeutics (DOI: 10.1124/jpet.118.252320), UCT’s octadecylsilane (C18) sorbent was employed to investigate the possible role of the endocannabinoid system in Reno medullary interstitial cells.
In the method, the culture medium was extracted using Clean-Up® C18 solid phase extraction columns (500 mg). Briefly, the. SPE columns were conditioned with 3 mL of methanol followed by 3 mL of deionized water. The samples of culture medium (25 mL) were then passed through the columns and washed, first using 3 mL of DI water followed by 3 mL of 25% methanol and 3 mL of 60% methanol. The lipids eluting with 100% methanol were collected and evaporated under nitrogen leaving ∼500 µL. 50 µL was transferred to auto-sampler vials for reverse phase HPLC-UV analysis.
When biopharmaceutical scientists working on clinical studies require ultra-pure sorbents that can assist in extracting materials from culture media, they turn to UCT as their first choice.