Identifying antibodies (Abs) that neutralize infectious agents is the first step for developing therapeutics, vaccines, and diagnostic tools for infectious agents. However, current approaches for identifying neutralizing Abs (nAbs) typically rely on dilution-based
assays that are costly, inefficient, and only survey a small subset of the entire repertoire.
In a recent paper authored Jose A. Wippolda et al., (Lab an a Chip (DOI: 10.1039/C9LC01165J) the authors describe the development of PRESCIENT (Platform for the Rapid Evaluation of antibody SucCess using Integrated microfluidics ENabled Technology), a droplet microfluidic system that can enable high-throughput single-cell resolution identification of nAb repertoires elicited in response to a viral infection.
Integral to the process was the use of UCT Specialty Chemical (tridecafluoro-1,1,2,2 tetrahydrooctyl) trichlorosilane to coat a photoresist for 20 min using a conventional desiccator, to prevent pattern removal during polymethyldimethylsilane (PDMS) removal
With their instrument, the authors demonstrated PRESCIENT’s ability to identify Abs that neutralize a model viral agent, Murine coronavirus (Murine Hepatitis Virus), which causes high mortality rates in experimentally infected mice.
In these uncertain days of novel viruses, UCT is proud to support front line scientists in their studies by providing the very finest in specialty chemicals. For more information regarding UCT Specialty Chemicals, applications and methods please visit https://www.unitedchem.com/product-category/silane/