UCT Silane used in Cellular Modeling

November 9th, 2016 Sample Preparation News

In the field of bio-mechanical research, scientists working in the field of cellular modeling require the finest of specialty chemicals in order to produce world class studies. UCT Inc., is known around world as a premier manufacturer of Silanes and Siloxanes. It is known though cellular modeling that cells sense their physical environment and convert biophysical cues into intracellular signals, which results in regulation of adhesion, morphology, and differentiation characteristics.  It has been shown in research studies that substrate properties, such as stiffness, control cell morphology, elongation, alignment (spatial orientation), and anisotropy of cell, and nucleus shape, partially direct the cell fate. Thus, it is of utmost importance to achieve elongated and aligned cellular organization in vitro for functional and hierarchical study of cell behavior.

Previously, methods exploiting substrate topography were utilized to achieve cellular alignment, using techniques such as electrospinning,  electrochemical compaction, and microfabricated grooves and pillars.  Even though these approaches have advanced our understanding of cell–substrate interactions, they are limited to 2D planar substrate. In a paper by Alapan et al (Case Western Reserve University, OH, USA) published in Advanced Healthcare Materials  (Adv. Healthcare Mater. 2016, 5, 1884–1892)  Micropillar Fabrication: Micropillar arrays were fabricated based on photolithography and soft lithography. A positive template on a silicon wafer, consisting an array of pillars, was fabricated using photolithography and wet chemical development. Positive template was silanized with (tri-deca fluoro-1,1,2,2-tetrahydrooctyl)-1-trichlorosilane) vapor under vacuum to facilitate separation of molded polymers  from the template. In this study involving Anisotropically Stiff 3D Micropillar Niche Induces Extraordinary Cell Alignment and Elongation, cellular modeling was achieved.  This research shows that when bio mechanical scientists  require the best specialty chemicals for their modeling they turn to UCT.

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