SELECTRAZYME-0

SELECTRAZYME β-Glucuronidase

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Abalone derived β-glucuronidase is used for the enzymatic hydrolysis of glucuronides from urine, blood and serum prior to analysis by enzyme immunoassay, mass spectrometry, high performance liquid chromatography, and other means. Typically, between 1 to 10 units of glucuronidase is used per microliter of the sample matrix. The exact amount needed will depend on the specific conditions used and must be determined empirically.

Abalone derived β-glucuronidase is a crude solution of enzymes. Many β-glucuronidases derived from mollusks also contain sulfatase activity. For this reason, the sulfatase activity of the material is also determined. Abalone derived β-glucuronidase is more thermal tolerant as compared to enzymes derived from E. coli, H. pomatia and bovine liver. Therefore the hydrolysis reaction can be carried out at a higher temperature providing hydrolysis in less time and achieving a higher degree of hydrolysis of metabolites like morphine-3-glucuronide.

Another application of Abalone derived β-glucuronidase is for the deconjugation of steroids in human and animal urine samples. When compared with H. pomatia, Limpet and bovine liver, abalone derived β-glucuronidase provides best results. For reference to this study, please site “Enzymatic Hydrolysis of Conjugated Steroid Metabolites: Search for Optimum Conditions Using Response Surface Methodology” V. Ferchaud, P. Courcoux, B. LeBizec, F. Monteau & F. Andre, The Analyst, 2000, 125, 2255-2259.

* All enzyme products will ship priority overnight on ice regardless of the chosen shipping option upon check out. This maximizes stability of the reagent.

Order SELECTRAZYME β-Glucuronidase

ImagePart NumberDescriptionCategoriesSizeUnitsPriceBuyhf:categorieshf:att:pa_sizehf:att:pa_column
BETA-GLUC-1MUBeta-Glucuronidase Enzyme 1,000,000 fishman unitsSELECTRAZYME β-Glucuronidase1,000,0001$151.00
selectrazyme-beta-glucuronidase derivatizingreagents-betaglucuronidase4b-1_000_000
BETA-GLUC-10Beta-Glucuronidase Enzyme 10SELECTRAZYME β-Glucuronidase101$216.00
selectrazyme-beta-glucuronidase derivatizingreagents-betaglucuronidasea1-10
BETA-GLUC-100Beta-Glucuronidase Enzyme 100SELECTRAZYME β-Glucuronidase1001$1,066.00
selectrazyme-beta-glucuronidase derivatizingreagents-betaglucuronidase23-100
BETA-GLUC-2MUBeta-Glucuronidase Enzyme 2,000,000 fishman unitsSELECTRAZYME β-Glucuronidase2,000,0001$271.00
selectrazyme-beta-glucuronidase derivatizingreagents-betaglucuronidase72-2_000_000
BETA-GLUC-25Beta-Glucuronidase Enzyme 25SELECTRAZYME β-Glucuronidase251$335.00
selectrazyme-beta-glucuronidase derivatizingreagents-betaglucuronidasefa-25
BETA-GLUC-250KUBeta-Glucuronidase Enzyme 250,000 fishman unitsSELECTRAZYME β-Glucuronidase250,0001$76.00
selectrazyme-beta-glucuronidase derivatizingreagents-betaglucuronidase60-250_000
BETA-GLUC-50Beta-Glucuronidase Enzyme 50SELECTRAZYME β-Glucuronidase501$595.00
selectrazyme-beta-glucuronidase derivatizingreagents-betaglucuronidasec5-50
BETA-GLUC-500KUBeta-Glucuronidase Enzyme 500,000 fishman unitsSELECTRAZYME β-Glucuronidase500,0001$103.00
selectrazyme-beta-glucuronidase derivatizingreagents-betaglucuronidasef5-500_000