UCT is widely recognized as the premier manufacturer of specialty chemicals by researchers working in the area of biological analysis and modeling. This is exemplified by a paper published in Biomaterials by X.Guo et al., ((2017) 122 179-187). In their study, they report the de novo induction of human intrafusal skeletal muscle fibers from satellite cells and their synaptic connection with human stem cell-derived sensory neurons in a defined in vitro system. Through their connection with proprioceptive sensory neurons, sensation of axial body position and muscle movement are transmitted to the central nervous system. Impairment of this sensory circuit causes motor deficits and has been linked to a wide range of diseases. As an integral part of the study, process microscope coverslips were coated with UCT’s silane trimethoxysilylpropyldiethylenetri-amine (DETA, T2910KG) and were used to produce a film by the reaction of the cleaned surfaces with a 0.1% (v/v) mixture of the organosilane in freshly distilled toluene. This film was employed to study the induction of intrafusal fibers which were characterized by morphology and fiber-type specific transcription factor expression using phase microscopy and immunocytochemistry.
The connections between sensory neurons and the intrafusal fibers were observed morphologically by type-specific innervation. To date, no defined human-based in vitro model of the proprioceptive sensory circuit has been developed. The goal of this study was to develop a human-based in vitro muscle sensory circuit utilizing human stem cells. When co-cultured with proprioceptive sensory neurons derived from human neuroprogenitors, mechanosensory nerve terminal structural features with intrafusal fibers were demonstrated. This article demonstrates why researchers working at the cutting edge of biological studies turn to UCT for the finest of specialty chemicals. For more information regarding UCT’s specialty chemical line including silanes similar to those used in the above study, visit https://specialties.unitedchem.com/products.