SELECTRAZYME β-Glucuronidase

Abalone derived β-glucuronidase is used for the enzymatic hydrolysis of glucuronides from urine, blood and serum prior to analysis by enzyme immunoassay, mass spectrometry, high performance liquid chromatography, and other means. Typically, between 1 to 10 units of glucuronidase is used per microliter of the sample matrix. The exact amount needed will depend on the specific conditions used and must be determined empirically.

Abalone derived β-glucuronidase is a crude solution of enzymes. Many β-glucuronidases derived from mollusks also contain sulfatase activity. For this reason, the sulfatase activity of the material is also determined. Abalone derived β-glucuronidase is more thermal tolerant as compared to enzymes derived from E. coli, H. pomatia and bovine liver. Therefore the hydrolysis reaction can be carried out at a higher temperature providing hydrolysis in less time and achieving a higher degree of hydrolysis of metabolites like morphine-3-glucuronide.

Another application of Abalone derived β-glucuronidase is for the deconjugation of steroids in human and animal urine samples. When compared with H. pomatia, Limpet and bovine liver, abalone derived β-glucuronidase provides best results. For reference to this study, please site “Enzymatic Hydrolysis of Conjugated Steroid Metabolites: Search for Optimum Conditions Using Response Surface Methodology” V. Ferchaud, P. Courcoux, B. LeBizec, F. Monteau & F. Andre, The Analyst, 2000, 125, 2255-2259.

* All enzyme products will ship priority overnight on ice regardless of the chosen shipping option upon check out. This maximizes stability of the reagent.

For international ordering, contact your International Distributor.

For sample requests, please contact your Regional Sales Representative

Part # Description Size Units Price Quantity Purchase
BETA-GLUC-10 Beta-Glucuronidase Enzyme 10 10 mL 1 $206.00
BETA-GLUC-25 Beta-Glucuronidase Enzyme 25 25 mL 1 $319.00
BETA-GLUC-50 Beta-Glucuronidase Enzyme 50 50 mL 1 $567.00
BETA-GLUC-100 Beta-Glucuronidase Enzyme 100 100 mL 1 $1,015.00
BETA-GLUC-250KU Beta-Glucuronidase Enzyme 250,000 fishman units 250,000 units 1 $72.00
BETA-GLUC-500KU Beta-Glucuronidase Enzyme 500,000 fishman units 500,000 units 1 $98.00
BETA-GLUC-1MU Beta-Glucuronidase Enzyme 1,000,000 fishman units 1,000,000 units 1 $144.00
BETA-GLUC-2MU Beta-Glucuronidase Enzyme 2,000,000 fishman units 2,000,000 units 1 $258.00

Beta-Glucuronidase Brochure

Enzymes At a Glance+FAQ