The compound list for forensic and animal toxicologists to identify, confirm and quantify is constantly increasing. The potential for liquid chromatography-high resolution accurate mass (LC-HRAM) spectrometry to identify these said ‘unknown’ compounds using non-targeted screening methods provides a potential advantage in the fight against doping in biological samples. The need to extract these newer drugs cleanly and at the highest recoveries and lowest matrix effects is paramount for the toxicologists. In a recent paper published in Analytical Methods ((2016, 8: 1789) Cawley et al., used UCT’s Xtrackt ® SPE column to isolate 2,5-dimethoxy-N- (2-methoxybenzyl)phenethylamine (NBOMe) compounds from samples of equine urine in a validation study to develop a workflow procedure developed for non-targeted analysis using a commercial differential analysis software platform. Six NBOMe compounds (25B, 25C, 25D, 25E, 25H and 25I) were studied to develop and optimize the proposed non-targeted screening workflow before two additional candidates (25N and 25T2) were used as blind controls for verification. Chromatographic alignment and the integration threshold were found to be the most critical parameters for successful identification of ‘unknown’ responses. The proposed workflow serves as an example for anti-doping laboratories to implement fit for-purpose non-targeted screening methods. When forensic and veterinary toxicologists working at the cutting edge of research for newer drugs and poisons need the most reliable extraction sorbents, they turn to UCT.