The aldehyde-coated slides were prepared by adding glass slides to a 2 mM of UCT PSX1050 in toluene for 4 hours. The slides were removed from the solution, rinsed with toluene and dried in nitrogen. Solutions of lectin were prepared in varying concentrations and dispersed onto the slide surfaces. Con A and E.Coli samples were applied to the aldehyde-functionalized glass slide and incubated for one hour and then dried in nitrogen.
The microarray assays were introduced to a solution of glycol FSNPs in HEPES for 2 hours, rinsed and dried. The slides were scanned with a GenePix 4000B at 532 nm. The fluorescent images were recorded and the data analyzed for each array.
The work reported a 20% to 30% reduction in fluorescence after being UV radiated, when compared to an unradiated standard.
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