Numerous polymerase-chain-reaction (PCR) and isothermal amplification methods employed currently in molecular diagnostic assays have been developed, significantly improving assay sensitivity and specificity as well as shortening overall time. In spite of this objective, nucleic acid target amplification continues to present challenges for dealing with “false-positive” results due to environmental or reagent contaminants introduced during the testing process. This has been addressed in a recent article authored by Wanyuan Ao et al., published in PLoS ONE 12(2): e0171915 in which UCT silane amine-functional T-structure polydimethylsiloxane (TSPS) was described in the production of crystalline silicon wafers. In this paper, the authors describe a strategy that can distinguish between staphylococcus species truly present in a clinical sample from contaminating staphylococcus species introduced during the testing process.Continue Reading
In a new paper authored by Szabolcs Sofalvi et al., published in Journal of Analytical Toxicology ((2017) 41 473–483), a validated method for the extraction of fentanyl, norfentanyl, acetyl fentanyl, carfentanil, 2-furanyl fentanyl and 3-methylfentanyl was reviewed using Clean Screen® DAU and LC-MS/MS to identify these novel compounds along with their corresponding concentrations.
The analysis of hair provides forensic toxicologists with several significant benefits in casework. Not only is it utilized when more conventional matrices such as blood and urine are not available, but also it provides an approximate timeline of drug use. Hair analysis is particularly useful in cases of child custody, workplace drug testing or in drug facilitated sexual assault cases. In a new paper authored by Lorna A Nesbit et al., published in Journal of Analytical Toxicology (doi: 10.1093/jat/bkx053) a group of NPS compounds (25B-, 25C- and 4-iodo-2,5-dimethoxy-N-[(2-methoxyphenyl)methyl]-benzeneethanamine) and 25I-NBOMe, using 4-bromo-2,5-dimethoxy-N-[(2-methoxyphenyl)methyl]-benzeneethanamine (25B-NBOMe)-D3, were analyzed using UCT’s flagship SPE sorbent Clean Screen® DAU (CSDAU133).Continue Reading